Fig. 3

The effect of increasing NaCl (75, 150 and 300 mM) concentration in the absence and presence of BugBuster^® (BB) keeping the buffer volume constant at 1250 μL. a, b Induced pellets after 6 h of induction were either incubated with LSB-2 with or without BugBuster^® and increasing concentration of NaCl. Post-centrifugation, the pellets and supernatants were subjected to SDS-PAGE. c The total soluble protein concentration of the supernatants, both with or without BugBuster^® was estimated using Bradford’s assay and plotted. Note the 6 to 9-fold increase in the total soluble protein concentration in the supernatants when BugBuster^® is used. d The induced fusion protein band (the band of interest) of the SDS-PAGE gels were analyzed using the ImageJ tool, both with and without BugBuster^®. e Statistical analysis of the total soluble protein in the supernatants from treatments with and without BugBuster^®. BB, NSG and SG, respectively stand for BugBuster^®, significant and non-significant values as analyzed using one-way ANOVA and Tuckey method. Statistically significant difference between the group means F(5,12) = 2140.42, p = 2.89E⁻¹⁷, F_{critical value} = 3.10, wherein F statistic (between,df_within) = F ratio, p-value, F-critical value. The molecular weights of CrFAP65AH1 and CrFAP65AH1V12P is 29.4 kDa and that of CrFAP65AH2 and CrFAP65AH2V12P is 30.64 kDa. For both the gel images, the brightness and contrast have been adjusted to 20%