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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Strategic optimization of conditions for the solubilization of GST-tagged amphipathic helix-containing ciliary proteins overexpressed as inclusion bodies in E. coli

Fig. 2

Kinetics of induction of the recombinant proteins (a) and formation of insoluble fusion polypeptides (b). a Uninduced (UI) E. coli cell lysate and kinetics of induction of the recombinant proteins at 1, 3 and 6 h. b Uninduced (UI), induced (I) E. coli cells and protein fraction of pellet (P) and supernatant (S) after lysis followed by centrifugation. Both pellets and supernatants were then electrophoresed using 12% SDS-PAGE. The molecular weights of CrFAP65AH1 and CrFAP65AH1V12P is 29.4 kDa and that of CrFAP65AH2 and CrFAP65AH2V12P is 30.64 kDa. c Transmission electron microscopy images of E. coli BL21DE3: Uninduced E. coli BL21 DE3 cells, Induced CrFAP65AH1, Induced CrFAP65AH2, Induced GST, Induced CrFAP65AH1V12P, Induced CrFAP65AH2V12P. The brightness and contrast features for the gels have been adjusted between 12 and 16%

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Microbial Cell Factories

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