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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Strategic optimization of conditions for the solubilization of GST-tagged amphipathic helix-containing ciliary proteins overexpressed as inclusion bodies in E. coli

Fig. 1

a Cartoon representation of CrFAP65 primary sequence with the ASH domains (grey filled boxes) and amphipathic helices (yellow filled boxes) and the hydropathy plots (b, c) derived for all the four polypeptides without the GST tag. This was carried out using an in-silico tool (https://web.expasy.org/protscale/ and https://web.expasy.org/protparam/). Note the presence of 7 ASH domains and 2 amphipathic helices. d In silico parameters and helical wheel projection using HeliQuest (https://heliquest.ipmc.cnrs.fr/)

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Microbial Cell Factories

ISSN: 1475-2859

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