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Table 2 Alpha amylase activities of the crude enzyme from P. thermoglucosidasius TM333 under various assay conditions similar to SSF conditions used in this study

From: Simultaneous saccharification and ethanologenic fermentation (SSF) of waste bread by an amylolytic Parageobacillus thermoglucosidasius strain TM333

pH

Temperature °C

Activity *U/ml

Condition used

7.0

60

195.5 ± 3.5

P. thermoglucosidasius fermentation

7.0

85

428.5 ± 7.2

Liquefaction

7.0

100

8.1 ± 0.6

Gelatinisation

5.5

35

29.2 ± 1.4

Yeast fermentation

  1. One unit of α-amylase is the amount of enzyme that released reducing sugars equivalent to 1 µmole of maltose per minute under the assay conditions.
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Microbial Cell Factories

ISSN: 1475-2859

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