Fig. 3

In vivo overexpression of katG and ahpC/F and ROS-GloTM Assay signals from H₂O₂ production by strains. A Strains AI_2211, AI_2217, and AI_2218 were cultivated in the 10 ml defined medium with 10 ml dodecane in shaking flasks at 100 rpm and 300 rpm. The data were an average of duplicate data. B α-Ionone titers of AI_2211 and AI_2218 under 250 rpm or 500 rpm shaking speed by RTS-1C (Personal bioreactor) and after incubation 1.5 h, 100 µL of each testing strains AI_2211 and AI_2218 mixed culture plated in a 96-well white cell culture plate and 100 µL of ROS-GloTM Detection Solution was added to the wells. Luminescence was determined with a GloMax^® Multi + Luminometer. The average relative light unit (RLU) and standard deviation of quadruplicate samples were calculated. The titers and luminescence values were statistically analyzed by one-way ANOVA: ns, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001