Fig. 2

Lithoautotrophic cell growth curves of the engineered strains overexpressing A the heterologous RuBisCo (rbcLXS) and B the transcriptional regulators (cbbR and/or regA) under nitrogen-rich conditions (1 g/L (NH₄)₂SO₄). The gene of interest was induced by adding 0.2% (w/v) of l-arabinose after 24 h of culture. The C. necator H16 strain harboring pBAD empty vector was used as a control. The data represent the means of triplicate experiments