Table 4 Fermentation results for shake flask cultivations of A. oryzae with different initial product concentrations
Conditiona | Acetic acid, initial-finalb [g/L] | Malic acid, final-initial [g/L] | Malic acid, final-minimalc [g/L] | YP/S, malic acid, final-initial [g/g] | Productivity malic acid, final-initial [g/L/h] | Succinic acid, final-initial [g/L] | Fumaric acid, final [g/L] | Total acid, final-initial [g/L] | YP/S, total acid, final-initial [g/g] | Productivity total acid, final-initial [g/L/h] |
|---|---|---|---|---|---|---|---|---|---|---|
2 g/L M | 44.33 ± 0.38 | 7.18 ± 0.25 | 7.41 ± 0.12 | 0.16 ± 0.01 | 0.030 ± 0.001 | 5.75 ± 0.73 | 0.87 ± 0.03 | 14.34 ± 0.88 | 0.32 ± 0.02 | 0.060 ± 0.004 |
6 g/L M | 44.18 ± 1.63 | 6.92 ± 0.11 | 7.98 ± 0.15 | 0.16 ± 0.00 | 0.029 ± 0.000 | 5.69 ± 0.43 | 1.59 ± 0.05 | 14.82 ± 0.37 | 0.34 ± 0.02 | 0.062 ± 0.002 |
10 g/L M | 40.45 ± 1.28 | 5.88 ± 0.28 | 7.31 ± 0.26 | 0.15 ± 0.00 | 0.024 ± 0.001 | 4.92 ± 0.44 | 2.16 ± 0.03 | 13.90 ± 0.65 | 0.34 ± 0.01 | 0.058 ± 0.003 |
20 g/L M | 39.87 ± 3.19 | 5.51 ± 1.19 | 8.37 ± 0.99 | 0.14 ± 0.02 | 0.023 ± 0.005 | 5.28 ± 1.06 | 3.50 ± 0.15 | 15.19 ± 1.86 | 0.38 ± 0.02 | 0.063 ± 0.008 |
35 g/L M | 38.77 ± 3.10 | 4.56 ± 0.35 | 8.57 ± 0.37 | 0.12 ± 0.01 | 0.019 ± 0.001 | 5.98 ± 0.51 | 5.76 ± 0.09 | 16.90 ± 0.60 | 0.44 ± 0.02 | 0.070 ± 0.002 |
50 g/L M | 31.43 ± 1.54 | 2.79 ± 0.75 | 8.02 ± 0.51 | 0.09 ± 0.02 | 0.012 ± 0.003 | 5.83 ± 0.82 | 7.49 ± 0.12 | 16.56 ± 1.58 | 0.53 ± 0.03 | 0.069 ± 0.007 |
1 + 1 g/L M + S | 44.95 ± 0.09 | 8.23 ± 0.38 | 8.23 ± 0.38 | 0.18 ± 0.01 | 0.034 ± 0.002 | 6.36 ± 0.97 | 0.73 ± 0.01 | 15.78 ± 1.22 | 0.35 ± 0.03 | 0.066 ± 0.005 |
3 + 3 g/L M + S | 41.87 ± 2.62 | 6.91 ± 1.00 | 7.18 ± 1.04 | 0.16 ± 0.01 | 0.029 ± 0.004 | 4.59 ± 0.94 | 1.09 ± 0.11 | 13.29 ± 1.82 | 0.32 ± 0.02 | 0.055 ± 0.008 |
5 + 5 g/L M + S | 41.35 ± 6.19 | 7.24 ± 0.93 | 7.85 ± 1.17 | 0.18 ± 0.01 | 0.030 ± 0.004 | 5.14 ± 1.77 | 1.47 ± 0.10 | 14.49 ± 2.51 | 0.35 ± 0.01 | 0.060 ± 0.010 |
10 + 10 g/L M + S | 44.40 ± 0.69 | 8.28 ± 0.11 | 9.32 ± 0.14 | 0.19 ± 0.00 | 0.035 ± 0.000 | 7.52 ± 0.25 | 2.32 ± 0.06 | 18.83 ± 0.38 | 0.42 ± 0.00 | 0.078 ± 0.002 |
17.5 + 17.5 g/L M + S | 43.74 ± 2.00 | 7.36 ± 0.54 | 9.28 ± 0.42 | 0.17 ± 0.01 | 0.031 ± 0.002 | 7.59 ± 0.80 | 3.56 ± 0.15 | 19.48 ± 1.04 | 0.45 ± 0.01 | 0.081 ± 0.004 |
25 + 25 g/L M + S | 43.77 ± 1.97 | 6.73 ± 0.69 | 9.60 ± 0.58 | 0.15 ± 0.01 | 0.028 ± 0.003 | 8.10 ± 0.64 | 4.71 ± 0.05 | 20.43 ± 1.15 | 0.47 ± 0.02 | 0.085 ± 0.005 |
Control | 43.68 ± 2.80 | 7.99 ± 0.42 | 7.99 ± 0.42 | 0.18 ± 0.00 | 0.033 ± 0.002 | 5.63 ± 0.34 | 0.60 ± 0.02 | 15.03 ± 0.69 | 0.34 ± 0.01 | 0.063 ± 0.003 |