Fig. 4

Single-cell fluorescence data from cells detected as having a fluorescence emission value above non-fluorescent control, and thus retaining fluorescent protein expression from the production operon, throughout the 106 h continuous fermentation. A The percentage of cells exhibiting a fluorescence reading above negative control and thus the proportion that still retain fluorescent protein expression from the production operon. B The median fluorescence value of cells that retain a fluorescent signal above control, indicating the comparative, average amount of fluorescent protein molecules in individual cells. C The coefficient of variance of this median fluorescence value and thus the variation in amounts of fluorescence protein molecules in cells across this population sample