Fig. 4
From: A CRISPR-based chromosomal-separation technique for Escherichia coli
Stability of the chromosomal organization of genome-engineered E. coli strains. A Two genome-engineered E. coli strains were continuously transferred in batch culture at an inoculation ratio of 1‰ for 11 rounds, corresponding to approximately 100 generations. B, C Colony PCR analysis of the E. coli0.10/4.54 cells after culturing for more than 100 generations using the primer pairs f1/r1, f2/r2, f1/r2 and f2/r1. D, F Colony PCR analysis of the E. coli2.28/2.36 (mix with E. coliMut) after culturing for more than 100 generations using the primer pairs f3/r3, f4/r4, f3/r4 and f4/r3. E, G Colony PCR analysis of the E. coli2.28/2.36 (mix with E. coliMut) after culturing for more than 100 generations using the primer pairs f5/r5, f6/r6, f5/f6 and r5/r6