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Table 2 Comparison of fermentation results with FruR mutants and PHE01

From: Engineering of global transcription factor FruR to redirect the carbon flow in Escherichia coli for enhancing l-phenylalanine biosynthesis

Mutants

Percentage a

OD610

L-PHE (g/L)

FruRE173K

40

37.36 ± 0.25

9.06 ± 0.34

FruRA18P

22

36.50 ± 0.05

8.86 ± 0.62

FruRL3F−L170N

16

33.95 ± 1.05

7.79 ± 1.05

FruRS75I−V160E

10

34.43 ± 1.12

8.31 ± 0.67

FruRP129H

6

34.52 ± 2.15

8.46 ± 0.69

FruRI144T

2

35.12 ± 1.03

8.05 ± 0.42

FruRE72R−P128A

2

28.51 ± 1.35

7.69 ± 0.36

FruRR312G

2

27.35 ± 0.25

7.48 ± 0.47

PHE01b

–

36.06 ± 0.35

7.32 ± 0.35

  1. a“Proportion” refers to the percentage of different types of mutants in all 50 candidates (%)
  2. bMeans the strain PHE01is used as a positive control; The fermentations were performed with a single clone for each FruR-variant
  3. The average value ± standard deviation is based on three independent experiments
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Microbial Cell Factories

ISSN: 1475-2859

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