Fig. 6
Regulations of central metabolic pathway and aromatic amino acid synthesis by the catabolites repressor/activator, Cra (FruR), in E. coli FruRE173K mutant. The genes shown in aqua green boxes are under the control of FruR, some of which were identified by the experiments, and others were predicted by SELEX chip analyses or other prediction methods. The metabolite shown in a yellow box indicates the regulatory function of FruR is controlled by the intracellular concentration of the key metabolite, fructose-1,6-biphosphate. Green arrows indicate down-regulation, red arrows indicate up-regulation. Quantitative fold changes and corrected p-values are listed in Additional file 2: DataSet S2. Key genes for enzymes: Glucose-6-phosphate isomerase pgi, Fructose-1,6-bisphosphatase fbp, Phosphofructokinase pfkA, Fructose bisphosphate aldolase fbaA, Triose phosphate isomerase tpi, Glyceraldehyde 3-phosphate dehydrogenase-A gapA, Glyceraldehyde 3-phosphate dehydrogenase C gapC, Phosphoglycerate kinase pgk, Phosphoglycerate mutase M gpmM, Phosphoglycerate mutase A gpmA, Enolase eno, Pyruvate kinase I pykF, pyruvate kinase II pykA, Isocitrate lyase aceA, Malate synthase AaceB, Isocitrate dehydrogenase icd, 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase aroG/aroH/aroF, 3-Dehydroquinate synthase aroB, 3-Dehydroquinate dehydratase aroD, Shikimate 5-dehydrogenase aroE, Shikimate dehydrogenase ydiB, Shikimate kinase II aroL, Shikimate kinase I aroK, 5-enolpyruvyl shikimate-3-phosphate synthase aroA, Chorismate synthase aroC, Bifunctional chorismate/prephenate dehydratase pheA/tyrA, Aromatic-amino-acid aminotransferase tyrB, Component of histidinol-phosphate aminotransferase hisC, Aspartate aminotransferase aspC