Fig. 1
Library screening and characterization using an L-PHE biosensor. a Schematic illustration of the design of the L-PHE biosensor used in this study. The L-PHE biosensor (Pmtr) is composed of a weak TyrR box (l-tyrosine binding site), a strong TyrR box (L-PHE binding site), and a TrpR box (l-tryptophan binding site). The biosensor regulates the expression of an engineered reporter (RFP) and placed upstream of the RFP reporter. b Fluorescence normalized by OD610 related to concentration of L-PHE supplemented into the media. c Extracellular L-PHE normalized by OD610 related to fluorescence normalized by OD610 (mean values with standard errors, n = 3 technical replicates). The p-value showing a significant slope is from a two-side t-test performed on means values for the five different strains, e.g., W3110, PHE01, PHE03, PHE04, and PHE05