Table 1 Strains and plasmids used in this study

Strains

 S. albulus CICC 11022

Wild type strain, transformation host

 S. albulus Q-PL2

pls gene high expressing strain harboring pSET152-pro-rbs2-pls

 S. albulus Q-dPLS

pls gene knockout strain constructed in this study

 E. coli ET12567/pUZ8002

recE, dcm⁻, dam⁻, hsdS, Cm^r, Tet^r, Str^r, Km^r, non-methylating plasmid donor strain for intergeneric conjugation

 E. coli Trans5α

General cloning strain

Plasmids

 pSET152-pro-rbs2-pls

Apr^r, pSET152 carrying kasOp* promoter, RBS2, and pls gene

 pKC1139

Apr^r, temperature-sensitive shuttle vector for gene knockout

 pKC1139-dpls

pls gene knockout plasmid based on pKC1139