Fig. 1
From: Garvicin Q: characterization of biosynthesis and mode of action
Lactococcus sp. B1726 produces a heat-stable and protease sensitive bacteriocin. A Overlay agar plates of Lactococcus sp. B1726 colonies with L. innocua LMG2785 as indicator strain. The red arrow marks a single colony of Lactococcus sp. B1726 and the white arrow highlights a zone of inhibition surrounding Lactococcus sp. B1726. B Growth inhibition assay of serial twofold dilutions of supernatants (SN) of Lactococcus sp. B1726. Where indicated SN were treated with trypsin (Trp) or incubated at 80 °C for 15 min (heat). MRS medium served as negative control. L. innocua LMG2785 was used as indicator strain. Values are mean ± standard deviation of n = 3 independent cultures. C Silver stained SDS-gel (left) and zymogram (right) of RPC fractions (C7-C9) of Lactococcus sp. B1726 SN. The black arrow indicates the antimicrobial active peptide. M = Spektra™ Low range ladder