Fig. 2

Effect of cell amounts on the oxidation of 10 g/L 1,6-HD to AA via 6-HHA at 30 °C in 1 mL for 12 h by 2 mL (1.6 mg CDW), 4 mL (3.2 mg CDW) and 6 mL (4.8 mg CDW) of G. oxydans 50049 in 1 mL of 100 mM sodium phosphate buffer, pH 7 at 30 °C. A Effect of cell amount of G. oxydans DSM50049, and B initial pH on the oxidation of 10 g/L 1,6-HD to AA via 6-HHA in 1 mL at 30 °C. The cell amounts used were 1.6 mg, 3.2 mg and 4.8 mg cell dry weight from 2, 4 and 6 mL culture broth, respectively, in (A), and 3.2 mg CDW in (B). AA, 6-HHA, and residual 1,6-HD were measured after 12 h in (A) and after 24 h in (B). pH was not controlled during the reaction