Fig. 2

Expression of HTC in L. lactis depending as a function of induction time. A Impact of short times of induction on protein expression (2-, 3- and 4-h post-induction). B Impact of long time of induction on protein expression (4- and 20-h post-induction). Total membrane proteins (10 µg) were separated in a 12% SDS–PAGE and analyzed by Western blot performed using an HRP conjugate specific to the His-tag affinity tag. A positive control protein containing caveolin-1β (C + ; 15 µg) was loaded and the band at 75 kDa of the molecular weight from Biorad was used to estimate the expression levels of the recombinant protein. o,d,m correspond respectively to oligomer, dimer and monomer