Table 2 Strains, plasmids, and primers used in this study
Strains, plasmids, and primers | Significant properties | Source or purpose |
|---|---|---|
Strains | ||
 Escherichia coli DH5α | General cloning host | Transgen Biotech |
 M3 | Wild type Mycolicibacterium neoaurum TCCC 11u978 (MNR) | Tianjin University of Science and Technology Culture Collection Center (TCCC) |
 QC M3 | Deletion of KsdD in Mycolicibacterium neoaurum TCCC 11u978 | Lab of Systematic Microbiology and Biomanufacturing Engineering, Tianjin University of Science and Technology |
 QC M3-N | QC M3 containing plasmid pMV261-Nat | This work |
 QC M3-C | QC M3 containing plasmid pMV261-AraC | This work |
 QC M3-A | QC M3 containing plasmid pMV261-AccA1 | This work |
 QC M3-D | QC M3 containing plasmid pMV261-AccD1 | This work |
 QC M3- AC | QC M3 containing plasmid pMV261-AraC-AccA1 | This work |
 QC M3-ATetO | QC M3 containing plasmid pMV262-AccA1 | This work |
 QC M3-ATetOC | QC M3 containing plasmid pMV262-AccA1-AraC | This work |
Plasmids | ||
 pMV261 | Shuttle vector, hsp60, Kan R | Dr. W. R. Jacobs Jr |
 pMV262 | Shuttle vector, Tet O, Tet R, Kan R | This work |
 pMV261-Nat | Nat was connected to pMV261 | This work |
 pMV261-AraC | AraC was connected to pMV261 | This work |
 pMV261-AccA1 | AccA1 was connected to pMV261 | This work |
 pMV261-AccD1 | AccD1 was connected to pMV261 | This work |
 pMV262-AccA1 | AccA1 was connected to pMV262 | This work |
 pMV261-AraC-AccA1 | AraC and AccA1 were Series connected to pMV261 | This work |
 pMV262-AraC-AccA1 | Adding tet operon between AraC and Acca1 at pMV262-AraC-AccA1 | This work |
Primers | ||
 AccA1-f | gcggatccagctgcagaattcATGGTCAACGAACTCTTCCACAC | AccA1 amplification |
 AccA1-r | tacgtcgacatcgataagcttTCATCGTTGGGACTCCTTGC | AccA1 amplification |
 AccD1-f | gcggatccagctgcagaattcATGACGCATCGTGAAGCGC | AccD1 amplification |
 AccD1-r | tacgtcgacatcgataagcttTCACATCCTGAAGACGCCGT | AccD1 amplification |
 Nat-f | gcggatccagctgcagaattcATGACCGTCGATGTGGCCG | Nat amplification |
 Nat-r | tacgtcgacatcgataagcttTCAGTTCCCCAGCACGCG | Nat amplification |
 AraC-f | gcggatccagctgcagaattcGTGAATGCCCCTCGGCGT | AraC amplification |
 AraC-r | tacgtcgacatcgataagcttTCAGTTACCTCTCATCCATTCCAG | AraC amplification |
 TetO-f | ccgtggcgcggccgcggtaccGAATTCATAACTTCGTATAATGTATGCTATAC | TetO amplification |
 TetO-r | tcgaattctgcagctggatccCTGGATCCGCAATTGTCTTGG | TetO amplification |
 16S-RT-F | GTAGGGTCCGAGCGTTGTC | Quantitative RT-PCR |
 16S-RT-R | GCGTCAGTTACTGCCCAGAG | Quantitative RT-PCR |
 AraC-RT-F | TCCTCACCGGAAGTGTTCTC | Quantitative RT-PCR |
 AraC-RT-R | ATACGAGCGAACTCATCGGT | Quantitative RT-PCR |
 Nat-RT-F | GTCGCTACCTGTGTGACGTG | Quantitative RT-PCR |
 Nat-RT-R | CTGTACGTCTTCACCACCGA | Quantitative RT-PCR |