Fig. 4

Recombinant strain qRT-PCR analysis, growth status, and AD conversion ratio of WT, QC M3-C and QC M3-N. a qRT-PCR analysis of the expression variations of AraC and Nat. b Growth curves of WT, QC M3-C and QC M3-N. c Time courses of biotransformation ratio of AD by WT, QC M3-C and QC M3-N. The folded line corresponds to AD concentration ration (left y-axis) and the bar corresponds to AD concentration (right y-axis). Error bars indicate the standard deviation from three independent experiments and the levels of statistical significance are indicated as follows: *p < 0.05; **p < 0.01; ***p < 0.001; ns, indicates no significant difference (p > 0.05)