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Fig. 8 | Microbial Cell Factories

Fig. 8

From: Identification of a flavonoid C-glycosyltransferase from fern species Stenoloma chusanum and the application in synthesizing flavonoid C-glycosides in Escherichia coli

Fig. 8

Production of vitexin (2b) and isovitexin (2c) by bioconversion in E. coli cell. A A simplified scheme showing the biosynthetic pathway for vitexin (2b) and isovitexin (2c). B The production of 2b and 2c in E. coli EA3 and EA4. C HPLC analysis of the reaction products of E. coli strains EA3, EA4, and negative control (NC) fed with 100 μM naringenin. D Typical negative ion MS and MS2 spectra of products 2b and 2c. E The genetic modules of strain EA5 in operon form. F The quantification of 2b and 2c produced by strains EA4 and EA5 fed with 100 μM naringenin. Data presented in the form mean ± SD (n = 3); *p < 0.05 and **p < 0.01 (MS: mass spectrometry; MS2: tandem mass spectrometry)

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Microbial Cell Factories

ISSN: 1475-2859

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