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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Identification of a flavonoid C-glycosyltransferase from fern species Stenoloma chusanum and the application in synthesizing flavonoid C-glycosides in Escherichia coli

Fig. 3

C-glycosylation of 2-hydroxynaringenin (2) catalyzed by recombinant ScCGT1. A ScCGT1 catalyzed 2-hydroxynaringenin (2) to produce 2a, 2b, and 2c. B HPLC chromatograms of the glycosylation reactions. C Typical negative ion MS and MS2 spectra of product 2a, 2b, 2c and standards. D. The putative splitting way of 2a, 2b, and 2c on MS2. S1, apigenin (dehydrated substrate); 2a, 2-hydroxynaringenin C-glycoside; 2b, vitexin; 2c, isovitexin. (2, 2-hydroxynaringenin; Glc, UDP-glucose; vit, vitexin; isovit, isovitexin; STD: Standard; MS: mass spectrometry; MS2: tandem mass spectrometry)

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Microbial Cell Factories

ISSN: 1475-2859

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