Fig. 3

flCDKL5 production profile with pMAV and p79C monocistronic plasmids. a SDS-PAGE (left panel) and anti-CDKL5 Western blot (right panel) of P. haloplanktis TAC125 crude lysates after the expression of the flCDKL5 variant named 107 (B). Two plasmids were tested in comparison to a noninduced strain (NI): the D-galactose inducible plasmid pMAV and the IPTG-inducible plasmid p79C. b Comparison of the expression profile of 107 (B) with 107 (G). These flCDKL5 variants are identical in terms of residues composition, but their nucleotide sequences differ for the removal of an A-rich stretch which is causative of an abortive truncation when 107 (B) is translated. The time points indicated below refer to the hours after induction.