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Table 1: Properties of isolated strains

From: Identification of acetic acid sensitive strains through biosensor-based screening of a Saccharomyces cerevisiae CRISPRi library

Strain

RFP expression*

Growth**

Target gene

Description of target gene

E or RE***

#3

+

ns

QCR8

Subunit of the ubiquinol cytochrome-c reductase [40]

RE

#8

+

–

TIF34

Subunit of the eukaryotic translation initiation factor 3 (eIF3) [41]

E

#13

+

–

MSN5

Karyopherin involved in nuclear import and export of proteins, involved in nuclear export of Haa1 [37, 42]

RE

#15

=

ND

NDC1

Subunit of the transmembrane ring of the nuclear pore complex (NPC) [43]

E

#17

+

–

PAP1

Poly(A) polymerase [44]

E

#32

=

 

CBP2

Nuclear-encoded mitochondrial protein that binds to RNA to promote splicing [45]

RE

#33

+

–

COX10

Heme A farnesyltransferase [46]

RE

#35

+

–

TRA1

Subunit of Spt–Ada–Gcn5 acetyltransferase (SAGA) and NuA4 histone acetyltransferase complexes [47]

E

#37

=

ns

UBA2

Subunit of heterodimeric nuclear small ubiquitin-like modifier protein (SUMO) [48]

E

#43

=

ND

RPS30B

Protein component of the small (40S) ribosomal subunit [49]

E

#46

=

ND

HSH49

U2-small nuclear ribonucleoprotein (snRNP) associated splicing factor [50]

E

#49

=

ND

LCB1

Component of serine palmitoyltransferase [51]

E

  1. *Reporter expression 30% higher (+) or similar (=) compared to the CBL
  2. **Growth significantly (p < 0.05) decreased (-) or not significantly different (ns) compared to the CC23 control strain. ND = not determined
  3. ***Essential (E) and respiratory growth essential (RE) genes are indicated