Fig. 6

Characterization of selected isolates at 150 mM acetic acid, pH 4.5. a Growth of control strain CC23 (red lines) and strains isolated from the CRISPRi library (green lines) measured as scattered light and b maximum fluorescence intensity (FI) of the biosensor normalized by biomass (red bars) and sfpHluorin ratio at the time of maximum biosensor output (green bars). The solid lines mark the average normalized FI or sfpHluorin ratio value of the CC23 control strain, the dashed lines mark a 33% increase in normalized FI or sfpHluorin ratio compared to CC23. c Maximum fluorescence intensity (FI) of the biosensor plotted against the sfpHluorin ratio of strains at the time of maximum biosensor output. Data obtained from six (CC23) or three biological replicates; whiskers and shadows show the standard deviation Statistical significance among growth curves was calculated using the “compareGrowthCurves” permutation test in R [52]; *p < 0.05