Fig. 3

Screening the CRISPRi library through FACS. a Growth of the CBL pool at 0 mM (black line) and 50 mM (red line) acetic acid. Time and OD₆₀₀ value at the sampling for FACS are annotated with red dotted lines. Data obtained from two biological replicates; shadowed regions show the standard deviation. b The most fluorescent cells were selected by FACS, resulting in 94 isolates and a pooled culture of 50,000 cells (TOP pool). c The distribution of fluorescence of the CBL cells at the initial FACS. The gate set for the sorting is represented by the red area. d The distribution of fluorescence of the pooled CBL cells (red area) compared to the distribution of fluorescence of cells selected through two rounds of FACS (blue area)