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Table 1 The constructed plamids for optimizing the interaction between F3′Hs and CPRs

From: Engineering Saccharomyces cerevisiae for the production of dihydroquercetin from naringenin

Constructed plasmids

Characteristics

Reference

pY26-SmF3′H-SmCPR

Episomal expression vector, containing the genes of SmF3′H and SmCPR from S. marianum

This study

pY26-SmF3′H-AtCPR

Episomal expression vector, containing the genes of SmF3′H from S. marianum and AtCPR from A. thaliana

This study

pY26-SmF3′H-EbCPR

Episomal expression vector, containing the genes of SmF3′H from S. marianum and EbCPR from E. breviscapus

This study

pY26-SmF3′H-GmCPR

Episomal expression vector, containing the genes of SmF3′H from S. marianum and GmCPR from G. max

This study

pY26-SmF3′H-HtCPR

Episomal expression vector, containing the genes of SmF3′H from S. marianum and HtCPR from H. tuberosus

This study

pY26-SmF3′H- HtCPR(L1M)

Episomal expression vector, containing the genes of SmF3′H from S. marianum and HtCPR from H. tuberosus(replaced the start codon UUG with ATG)

This study

pY26-SmF3′H- HtCPR(+ M)

Episomal expression vector, containing the genes of SmF3′H from S. marianum and HtCPR from H. tuberosus(added with a start codon of ATG)

This study

pY26-SmF3′H-ScCPR

Episomal expression vector, containing the genes of SmF3′H from S. marianum and HtCPR from S. cerevisiae

This study

pY26-FaF3′H-SmCPR

Episomal expression vector, containing the genes of FaF3′H from F. x ananassa and SmCPR from S. marianum

This study

pY26-FaF3′H-AtCPR

Episomal expression vector, containing the genes of FaF3′H from F. x ananassa and AtCPR from A. thaliana

This study

pY26-FaF3′H-EbCPR

Episomal expression vector, containing the genes of FaF3′H from F. x ananassa and EbCPR from E. breviscapus

This study

pY26-FaF3′H-GmCPR

Episomal expression vector, containing the genes of FaF3′H from F. x ananassa and GmCPR from G. max

This study

pY26-FaF3′H-HtCPR

Episomal expression vector, containing the genes of FaF3′H from F. x ananassa and HtCPR from H. tuberosus

This study

pY26-FaF3′H-ScCPR

Episomal expression vector, containing the genes of FaF3′H from F. x ananassa and ScCPR from S. cerevisiae

This study