Fig. 2
From: Engineering Saccharomyces cerevisiae for the production of dihydroquercetin from naringenin
Increasing SmF3′H’s stability by mutating potential ubiquitination sites. The potential ubiquitination sites on the enzyme SmF3′H were mutated based on the predicted results from BDM-PUB, and were episomlly expressed in a S. cerevisiae strain that integrated a copy of ScCPR into the genome HO site. The recombination strains were tested for their ability to convert NAR to ERI in a 24 deep-well microplate after 72-h fermentation. All experiments were performed in triplicates and error bars indicate SD