Fig. 1
From: Engineering Saccharomyces cerevisiae for the production of dihydroquercetin from naringenin
Plasmid construction and optimization of interaction between the flavonoid 3′-hydroxylase and the redox partner. A Schematic representation of DHQ bioproduction from NAR. B Plasmid construction for F3′H and CPR co-expression. C The tests for the optimal enzyme complex for NAR biotransformation. All the genes encoding the F3′Hs and CPR from different sources were controlled under the promoters of PINO1 and PTDH1, respectively. For testing the expressed enzyme complex activity, one gram of NAR was used as the substrate and added to the medium YPD. All experiments were performed in triplicates and error bars indicate SD