Table 1 Strains, plasmid, and primers used in this work

NT1259

W3110 ΔtrpL trpE ^FBR (M293T) N168D A478T C237R ΔtrpR::FRT ΔtnaA::FRT ΔsdaB::FRT Δlac:: P_tac-aroF-B-L Δfuc::P_tac-tktA Δxyl::P_tac-serA^FBR (T372N) Δrib::P_tac-trpB-trpA

[42]

NT1259 shiA_Cg

NT1259 mal::shiA_Cg

[43]

NT1405

NT1259 ara::P_tac-IGPs-myc-t

This work

NT1438

NT1259 ∆mal::P_tac-trpB-A

This work

NT1439

NT1405 ∆mal::P_tac-trpB-A

This work

NT1444

NT1439 ∆mtl::P_tac-serB

This work

NT1445

NT1439 ∆recA::P_t5-aroB

This work

NT1446

NT1445 ∆mtl::P_tac-serB

This work

pF112aroFBL_Kan

kan^R colE lacI^q P_tac-aroFBL

[7]

pCas

RepA101(Ts) kan P_cas-Cas9 P_araB-Red lacI^q P_trc-sgRNA-pMB1

[55]

pTarget-cat

pMB1 cat

[7]

pTarget-cat-_sgRib

pMB1 cat sgRNA-Rib

This work

pTarget-cat-_sgAra

pMB1 cat sgRNA-Ara

This work

pTarget-cat-_sgMal

pMB1 cat sgRNA-Mal

This work

pTarget-cat-_sgRec

pMB1 cat sgRNA-Rec

This work

pJF119-trpBA

ColE1 amp;

[7]

pJNNmod-trpC_mt

ColE1 amp; the synthesized fragment trpC (Mycobacterium tuberculosis) cloned into NdeI/HindIII sites under control of P_tac-promoter

This work

pJNNmod-serB

ColE1 amp; the PCR fragment serB cloned into NdeI/BamHI sites under control of P_tac-promoter

This work

pJ-P_T5-aroB

ColE1 amp; the PCR fragment RBS-aroB was cloned into EcoRI/BamHI sites, P_tac exchange to P_T5

This work

sg-reverse

ACTAGTATTATACCTAGGACTGAGCTAGC

rib-integration1

ACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATATCAAGGCGCACTCCCGTTCTGG

rib-integration2

ATTCACGCTAGCCCATACACCACGACTTCCTAAAGTAATCAGTACAGTACGGATACCCAGGGTTATTGTCTCATGAGCG

sgRib

GTATCGGTCAGCGCATCGGTGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAG

ara-integration1

TCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCGTCGCTCAAGGCGCACTCCCGTTCTGG

ara-integration2

ATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCCGTCAGGATGGCCTTCTGCTTAATTTGATGCC

sgAra

GTCGCCAGCTTGATTGATCTGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAG

mal-integration1

AAGGTAAACTGGTAATCTGGATTAACGGCGATAAAGGCTATAACGGTCTCGCTGTCAAGGCGCACTCCCGTTCTGG

mal-integration2

GATCGGTAATGCAGACATCACGGCAGCGGCGGCAAAGTCACCCCACAGGTAGTTTTCAGGGTTATTGTCTCATGAGCG

sgMal

GTTGCCATCCGGCAGAATGGGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAG

recA-integration1

TATAATTGCTTCAACAGAACATATTGACTATCCGGTATTACCCGTATCAAGGCGCACTCCCGTTCTGG

recA-integration2

GACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCCCAGGGTTATTGTCTCATGAGCG

sgRec

CCCAGTTTACGTGCGTAGATGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAG

mgsA-RT-5'

GCATTTAGTGGATCCCAGAAGAAAATC

mgsA-RT-3'

AACGGCATCAACCGTTACTGGAAC

gloA-RT-5'

GCGTGGATAAATACGAACTCGGCAC

gloA-RT-3'

CAATTTTGTAACCGTCCGGATCTTCCAC

gloB-RT-5'

GTCCCTTCAAACAACCGACCACAC

gloB-RT-3'

GTCCACAAGAGACACAAGATAAGGGAAC

gloC-RT-5'

GCGGAACTGGCGCAACATTACG

gloC-RT-3'

CCTGTAAAGTCACATTCCCTATGCTG

yeiG-RT-5'

CATTACCGGATGTATGATTATCTGCGCG

yeiG-RT-3'

GCACGGATTCACAATTGGCGCAAAGG

dld-RT-5'

CGCGCGTATAAATGAAGACGGCAAAC

dld-RT-3'

CGGGCGTGTCGGCTTCAATATCAC

yhbO-RT-5'

AAAAGGAGAAGCCAGCGTGACCATCG

yhbO-RT-3'

TGATCAGCAACTGCGGGCCGTGAC

yajL-RT-5'

ACGATACGCCCGGAACGGTGGAACTG

yajL-RT-3'

CAGCGATGGTAACCTGGCGATTACC

prs-RT-5'

ACAACGCCGCCGATGTCCGGAG

prs-RT-3'

ACTGCGAAAGTGGTTGCAGACTTCC

aroF-RT-5ʹ

GCTGCAGATCGCGCGTAAATTGCTG

aroF-RT-3ʹ

CCGGAGGCCATTTCACGGTGAG

trpC-mt-RT-5'

TCTGGATAGCATTCTGGAAGGTGTTC

trpC-mt-RT-3'

GCTTGCACGTTTAACTTCGGCAATAAC

trpC-ec-RT-5ʹ

CTTATGACGCGGGCGCGATTTACGG

trpC-ec-RT-3ʹ

CCAGCGATAACACCTTAGCTTTGTCC

serB-RT-5'

AAGTAACCGAACGGGCGATGCG

serB-RT-3'

CCGGAGGCAATCGCCACTTTC

aroB-RT-5ʹ

GCATGGTCGCGATACTACGCTGG

aroB-RT-3ʹ

CGCGCCAATCATGTTTTTACCGAGG

trpB-RT-5ʹ

GTTTTACCCATCCGCTTCGCCAGCAAC

trpB-RT-3ʹ

AATTTCAGGCTCAGTTCAACGACCTGCTG