Fig. 8From: Metabolic control analysis enables rational improvement of E. coli l-tryptophan producers but methylglyoxal formation limits glycerol-based productionSchematic representation of the main methylglyoxal formation and degradation pathway in Escherichia coli [51] A and RT-qPCR analysis of relative gene expression (without unit) of genes mgsA (B), gloA, gloB, gloC, yeiG, dld (C), yajL, and yhbO (D) relative to ftsZ gene of samples from the reference l-tryptophan production process with NT1446 pF112aroFBLKan after process times of 27Â h (R 27Â h), 43Â h (R 43Â h), 50Â h (R 50Â h), 55Â h (R 55Â h), and 70Â h (R 70Â h). IPTG for the induction of inducible genes was added 44Â h after inoculationBack to article page