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Fig. 8 | Microbial Cell Factories

Fig. 8

From: Metabolic control analysis enables rational improvement of E. coli l-tryptophan producers but methylglyoxal formation limits glycerol-based production

Fig. 8

Schematic representation of the main methylglyoxal formation and degradation pathway in Escherichia coli [51] A and RT-qPCR analysis of relative gene expression (without unit) of genes mgsA (B), gloA, gloB, gloC, yeiG, dld (C), yajL, and yhbO (D) relative to ftsZ gene of samples from the reference l-tryptophan production process with NT1446 pF112aroFBLKan after process times of 27 h (R 27 h), 43 h (R 43 h), 50 h (R 50 h), 55 h (R 55 h), and 70 h (R 70 h). IPTG for the induction of inducible genes was added 44 h after inoculation

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