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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Metabolic control analysis enables rational improvement of E. coli l-tryptophan producers but methylglyoxal formation limits glycerol-based production

Fig. 3

Gene expression (without units, relative to ftsZ control) of genes serB, aroF, aroB, trpC, trpCmt and trpB relative to ftsZ gene of samples from reference L-tryptophan production process after 27 h (R 27 h), 43 h (R 43 h), 50 h (R 50 h), 55 h (R 55 h) and 70 h (R 70 h) process time (IPTG for the induction of inducible genes was added 44 h after inoculation. The samples were taken from the production process with NT1446 pF112aroFBLKan (NT1446) and likewise with NT1259 shiACg pF112aroFBLKan (NT1259 shiA) [43]. Sample timepoints for NT1259 shiACg pF112aroFBLKan were the same with maximum deviations of 1.5 h. Results for NT1259 shiACg pF112aroFBLKan have been already published in [43]

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