Table 1 Strains, plasmids, and primers used in this study
Strain/plasmid/primer | Description | References/source |
|---|---|---|
Strains | ||
 E. coli DH5α | F − ϕ80d, lacZΔM15, endA1, recA1, hsdR17(rK − mK−), supE44, thi-1, gyrA96, relA1, Δ(lacZYA-argF)U169 | Invitrogen |
 S. cerevisiae CEN.PK2-1D | MATα ura3-52 leu2-3,112 trp1-289 his3Δ MAL2-8c SUC2 |  |
S. cerevisiae WT | S. cerevisiae CEN.PK2-1D harboring pRS423GPD, pRS424GPD, pRS426GPD | This study |
S. cerevisiae FC | S. cerevisiae CEN.PK2-1D harboring pRS423GPD_fucA, pRS424GPD_fucIU, pRS426GPD_fucK | This study |
 S. cerevisiae FCT | S. cerevisiae CEN.PK2-1D harboring pRS423GPD_fucA, pRS424GPD_fucIU, pRS426GPD_fucK_HXT4 | This study |
S. boulardii (ATCC MYA-796) | MATα ura3-52 leu2-3,112 trp1-289 his3Δ MAL2-8c SUC2 | [36] |
 S. boulardii WT | S. boulardii harboring pRS423GPD, pRS424GPD, pRS426GPD | This study |
 S. boulardii FCT | S. boulardii harboring pRS423GPD_fucA, pRS424GPD_fucIU, pRS426GPD_fucK_HXT4 | This study |
 HXT-null S. cerevisiae | S. cerevisiae D452-2 with a xylose pathway and deletion of HXT1-HXT7 | [42] |
 S. cerevisiae HXT1 | Hxt-null S. cerevisiae with overexpression of HXT1 | [42] |
 S. cerevisiae HXT2 | Hxt-null S. cerevisiae with overexpression of HXT2 | [42] |
S. cerevisiae HXT3 | Hxt-null S. cerevisiae with overexpression of HXT3 | [42] |
S. cerevisiae HXT4 | Hxt-null S. cerevisiae with overexpression of HXT4 | [42] |
 S. cerevisiae HXT6 | Hxt-null S. cerevisiae with overexpression of HXT6 | [42] |
S. cerevisiae HXT7 | Hxt-null S. cerevisiae with overexpression of HXT7 | [42] |
Plasmids | ||
 prs423GPD | HIS3, GPD promoter, CYC1 terminator, 2 µ origin, and Ampr | [43] |
 prs424GPD | LEU2, GPD promoter, CYC1 terminator, 2 µ origin, and Ampr | [43] |
 prs426GPD | URA3, GPD promoter, CYC1 terminator, 2 µ origin, and Ampr | [43] |
 prs423GPD_fucA | pRS423GPD harboring fucA from E. coli K12 MG1655 | This study |
 prs424GPD_fucIfucU | pRS424GPD harboring fucI and fucU from E. coli K12 MG1655 | This study |
 prs426GPD_fucKHXT4 | pRS426GPD harboring fucK from E. coli K12 MG1655 and HXT4 from S. cerevisiae CEN.PK2-1D | This study |
 prs426GPD_fucK | pRS426GPD harboring fucK from E. coli K12 MG1655 | This study |
Primers | ||
 F_Eco_fucA | 5′-ACTAGTATGGAACGAAATAAACTTGCTC-3′ | This study |
 R_Eco_fucA | 5′-CTCGAGTTACTCTTCAATTCGTAACC-3′ | This study |
 F_Eco_fucI | 5′-ACTAGTATGAAAAAAATCAGCTTACCGAA-3′ | This study |
 R_Eco_fucI | 5′-CTCGAGTTAACGCTTGTACAACGGAC-3′ | This study |
 F_Eco_fucU | 5′-ACTAGTATGCTGAAAACAATTTCGCC-3′ | This study |
 R_Eco_fucU | 5′-CTCGAGTTACGGTGTTACCCCTTTTT-3′ | This study |
 F_Eco_fucK | 5′-ACTAGTATGAAACAAGAAGTTATCCTGG-3′ | This study |
 R_Eco_fucK | 5′-CTCGAGTCACACTTCCTCTATAAATT-3′ | This study |
 F_Sce_HXT4 | 5′-ACTAGTATGTCTGAAGAAGCTGCCTATCAA-3′ | This study |
 R_Sce_HXT4 | 5′CTCGAGCTACTTTTTTCCGAACATCT-3′ | This study |