Table 1 S. cerevisiae strains used in this work
From: Development of an industrial yeast strain for efficient production of 2,3-butanediol
Strain | Genotype | Reference |
|---|---|---|
GSE16-T18HAA1 | Industrial 2G xylose-fermenting and inhibitor-tolerant strain | MCB, KULeuven [44] |
HGS1 | GSE16-T18 pdc1:: BDOp BDOp:2,3-BDO pathway being TDH3p-AlsD-ADH1t, ADH1p-AlsS-ADH2t, TEF1p-BdhA-CYC1t | This study |
HGS2 | HGS1 pdc6:: BDOp BDOp:2,3-BDO pathway being TDH3p-AlsD-ADH1t, ADH1p-AlsS-ADH2t, TEF1p-BdhA-CYC1t | This study |
HGS4 | HGS2 pdc5:: BDOp BDOp:2,3-BDO pathway being TDH3p-AlsD-ADH1t, ADH1p-AlsS-ADH2t, TEF1p-BdhA-CYC1t | This study |
HGS7 | HGS4 AD7:: TDH3p-BdhA-ADH1t, ADH1p-BdhA-ADH2t AD7, integration site | This study |
HGS21 | HGS7 mk114:: TDH3p-Aox1-ADH1t, ADH1p-Nde1-ADH2t, Mk114, integration site | This study |
HGS31 | HGS21 GPD2:: GPD2p-Nde1-GPD2t Nde1 replaces GPD2 | This study |
HGS38 | HGS31 GPD1:: GPD1p-Nde1-GPD1t Nde1 replaces GPD1 | This study |
HGS43 | HGS38 Mpc1 ∆ Mpc1 deletion | This study |
HGS48 | HGS43 Ora1∆ Ora1 deletion | This study |
HGS50 | HGS48 Mk20:: CYC1p-GPD1-GPD1t Mk20, integration site | This study |
HGS8 | HGS7 mk114:: TDH3p-Aox1-ADH1t Mk114, integration site | This study |
HGS15 | HGS8 GPD1∆, GPD2∆ GPD1 and GPD2 deletion | This study |
HGS17 | HGS8 GPD1p::CYC1p, GPD2∆ GPD1p replaced by CYC1p,GPD2 deletion |  |
HGS28 | HGS15 mk20:: CYC1p-GPD1-GPD1t Mk20,integration site | This study |
HGS29 | HGS28 Ora1 ∆ Ora1 deletion | This study |
HGS37 | HGS29 Mk119:: TEF1p-CSTL1-CYC1t Mk119, integration site | This study |