Fig. 6

Elimination of 2,3-dimethylglycerate production in the strain expressing the cytosolic NoxE enzyme and restoration of high glucose tolerance by increasing glycerol uptake. A Production of 2,3-BDO from 23% glucose in the strain HGS29 with deletion of ORA1. B Deletion of ORA1 eliminated 2,3-dimethylglycerate production. C Production of 2,3-BDO from 31% glucose in the strain HGS29 with deletion of ORA1. D Production of 2,3-BDO from 31% glucose in the strain HGS37 expressing the CaStl1 glycerol symporter. E Production of 2,3-BDO from 34% glucose in the strain HGS37 expressing the CaStl1 glycerol symporter. F Production of 2,3-BDO from 28% glucose in the strain HGS37 expressing the CSTL1 glycerol symporter