Fig. 4

Deletion of the MPC1 gene, encoding a mitochondrial pyruvate carrier subunit. A Production of 2,3-BDO from glucose, with supplementation of 0.5% ethanol, by strain HGS38 in which both GPD1 and GPD2 are replaced by NDE1. B Production of 2,3-BDO from glucose, with supplementation of 0.5% ethanol, by strain HGS43 in which MPC1 is deleted. C Production of 2,3-BDO from 100 g/L glucose in strain HGS48, which has a deletion of ORA1. D Deletion of ORA1 eliminated 2,3-dimethylglycerate production. E High initial glucose (210 g/L) compromises glucose utilization and 2,3-BDO production in strain HGS48. F Low expression of GPD1 restores complete utilization of a high initial glucose level (250 g/L) and supports production of a high 2,3-BDO titer in strain HGS50