Fig. 19
From: Novel prokaryotic system employing previously unknown nucleic acids-based receptors
Role of reverse transcriptase and integrase in the TC-system. A Effect of RTIs on bacterial growth and memory. Heat map representation of growth by control S. aureus, S. aureus TezR–D1d, S. aureus TezR–R1d, and S. aureus TezR–D1d/R1d upon treatment with RTIs. Nevirapine (NVP) and etravirine (ETR) were added to the broth and OD600 was monitored hourly for 6 h at 37 °C. OD600 is labeled by a color scale, from white (minimal) to red (maximum). Values show representative results of three independent experiments. B Biochemical profile of control B. pumilus grown on M9 minimal medium without (M9) or with dexamethasone (M9 + DEX), and with or without adding raltegravir (RAL). Green denotes positive test reaction results, red denotes negative results. Values show representative results of three independent experiments. C Raltegravir (RAL) added at different time points from 0 to 120 min to dexamethasone-sentient B. pumilus grown on M9 medium with dexamethasone (DEX). D Heat map showing the effect of raltegravir on signal transduction from TezRs in relation to temperature tolerance in control and raltegravir-treated (control + RAL) S. aureus (SA). CFU are labeled by a color scale, from white (minimum) to blue (maximum). Values show representative results of three independent experiments