Fig. 12

Effect of TezRs on B. pumilus chemotaxis to plasma and biofilm dispersal. A Control B. pumilus with no chemoattractant added. B Chemotaxis of control B. pumilus towards plasma as chemoattractant. C Chemotaxis of B. pumilus TezR–D1^d. D Biofilm dispersal and chemotaxis of B. pumilus TezR–R1^d. E Chemotaxis of B. pumilus TezR–D1^d/R1^d. F, H Chemotaxis and visibly expanded biofilm growth of B. pumilus TezR–D2^d and B. pumilus TezR–D2^d/R2^d. G, J Chemotaxis and intense biofilm dispersal of B. pumilus TezR–R2^d and B. pumilus TezR–R1^d/R2^d. I Chemotaxis of B. pumilus TezR–D1^d/D2^d. K Negative chemotaxis of B. pumilus TezR–D1^d/R1^d/D2^d/R2^d. Black dotted lines denote the area in which plasma was placed. The black arrow points to zones of active sporulation. A chemotactic response is visualized as a movement of the biofilm away from the center towards the chemoattractant. Representative images of three independent experiments