Table 1 The details and schematic representations of the used strains in this study
From: Exploring optimal Taxol® CYP725A4 activity in Saccharomyces cerevisiae
Strain name | Genotype | References |
|---|---|---|
LRS5 | MATa, leu2-3,112::HIS3MX6-pGAL1-ERG19/pGAL10-ERG8; ura3-52::URA3-pGAL1-MvaSA110G/pGAL10-MvaE (codon-optimised); his3Δ1::hphMX4-pGAL1-ERG12/pGAL10-IDI1;trp1-289::TRP1_pGAL1-CrtE(X. dendrorhous)/pGAL10-ERG20; YPRCdelta15::NatMX-pGAL1-CrtE(codon-optimised)/pGAL10-CrtE:: ARS1014a::pGAL1-TASY-GFP:: ARS1622:: pGAL1-MBP-TASY-ERG20*::ARS1114a::pGAL1-MBP-TASY-ERG20* | [12] |
LRS6 | LRS5 ARS511b::pGAL1-CYP725A4-PGK1t/pGAL3-POR-ENO2t; RKC3::pGAL1-TAT-CYC1t | [14] |
BN1 | LRS6 ARS416d::pGAL1-CYP725A4-PGK1t | This study |
BN2 | LRS6 ARS416d::pGal3-POR-ENO2t | This study |
BN3 | LRS5 ARS511b::pHXT7-CYP725A4-PGK1t/pGAL3-POR-ENO2t | This study |
BN4 | LRS6 ARS416d::pTRX2-CYP725A4-PGK1t/pGAL3-POR-ENO2t | This study |
BN5 | LRS5 X-2::pGAL1-CP725A4-RPL41Bt | This study |
BN6 | LRS5 X-2::pGAL1-CP725A4-RPL41Bt/pTDH3-POR-ICY2t | This study |
BNF-1 | LRS5 X-2::pGAL1-CP725A4-L1-POR-RPL41Bt | This study |
BNF-2 | LRS5 X-2::pGAL1-CP725A4-L2-EcfldA-L3-EcFpr-RPL41Bt | This study |
BNF-3 | LRS5 X-2::pGAL1-CP725A4-L4-AxRED(codon-optimised)-RPL41Bt | This study |
BNF-4 | LRS5 X-2::pGAL1-CP725A4-L5-L6-RhFRED(codon-optimised)-RPL41Bt | This study |
BNF-5 | LRS5 X-2::pGAL1-CP725A4-L7-BMR(codon-optimised)-RPL41Bt | This study |
BNF-6 | LRS5 X-2::pGAL1-CP725A4-AxRED(codon-optimised)-RPL41Bt | This study |
BNF-7 | LRS5 X-2::pGAL1-CP725A4-POR-RPL41Bt | This study |
BNF-8 | LRS5 X-2::pGAL1-CP725A4-truncated POR-RPL41Bt | This study |