Fig. 8

Resting cell assay of BN6 strain expressing Taxus cuspidata CYP725A4 and POR. A The concentration of terpenes and terpenoids by galactose induction A at the resting stage (post-rest induction) and B during the growth stage (pre-rest induction). The kinetic changes of relevant taxanes represent mean ± SD of triplicates over a five-day run of 115 h. The assays were both performed using 10 mL of 50 mM potassium phosphate buffer (pH = 7), containing 20% (v/v) dodecane. The starting biomass (OD₆₀₀) was 40 and the biomass accumulation was not changed by the end of 115 h. Iso.Taxadiene, t5alphaOl and iso.OCT represent iso-taxadiene, T5α-ol and iso-OCT. Figure was created with BioRender.com