Fig. 4

Screening the performance of BN6 strain expressing Taxus cuspidata CYP725A4 and reductase in tandem. A–G Taxane distribution and H biomass in different media in a 5-mL mini-scale for 72 h. Bars represent mean ± SD for triplicates. Tukey's HSD post hoc test shows a measure of difference across the whole strain groups, where different letters indicate significant differences of means (p < 0.05). The used media were: BHIG: Brain Heart Infusion Broth-2% (w/v) galactose; LBG: LB Broth (Lennox)-2% (w/v) galactose; SDG: Synthetic Defined-2% (w/v); YPG: Yeast Extract Peptone-2% (w/v) galactose; TBMG: Terrific Broth, Modified-2% (w/v) galactose. Iso.Taxadiene and iso.OCT represent iso-taxadiene and iso-OCT. Iso.Taxadiene, t5alphaOl, iso.OCT and Diterpenoid.I represent iso-taxadiene, T5α-ol, iso-OCT and diterpenoid I. Figure was created with BioRender.com