Fig. 3

Screening the performance of strains expressing self-sufficient fused CYP725A4 and reductases (BNF-1-7), self-sufficient fused CYP725A4 and transmembrane domain-truncated Taxus cuspidata reductase (POR) (BNF-8), separate CYP725A4 and T. cuspidata POR (BN6), CYP725A4 only (BN5), and parent taxadiene-producing LRS5 strain. A The strains were cultivated in 5 mL of YPG and A taxane products and B taxadiene and biomass accumulation were measured at the end of the 72 h. Bars represent mean ± SD for triplicates. Tukey's HSD post hoc test shows a measure of difference across the whole strain groups, where different letters indicate significant differences of means (p < 0.05). The asterisks indicate significant differences relative to LRS5 (for taxadiene, iso-taxadiene and OD₆₀₀) and BN5 (for taxanes) by pairwise t-test (***p < 0.001, **p < 0.01, *p < 0.05 adjusted p values)). Iso.Taxadiene, t5alphaOl and iso.OCT represent iso-taxadiene, T5α-ol and iso-OCT. Figure was created with BioRender.com