Table 2 Production of 5-HTP by microorganisms
Host | Hydroxylases | Cofactors | Cultivation mode | medium type | Titer (g/L) | Reference |
|---|---|---|---|---|---|---|
E. coli | Phenylalanine 4-hydroxylase form Chromobacterium violaceum with mutations of L101Y and W180F | BH4 | Shake flask; Supplementation of 5 mM L-Trp | - | 0.55 | Hara et al., 2013 [11] |
E. coli | Phenylalanine-4-hydroxylase from Xanthomonas campestris with a mutation of W179F | MH4 | Shake flask; Supplementation of 2 g/L L-Trp | M9 minimal medium | 1.1–1.2 | Lin et al.,. 2014 [16] |
E. coli | Aromatic amino acid hydroxylase from Cupriavidus taiwanensis with a mutation of W179F | BH4 | Supplementation of 1 g/L L-Trp | Mineral medium | 0.55 | Mora-Villalobos et al., 2017 [44] |
E. coli | Aromatic amino acid hydroxylase from Cupriavidus taiwanensis with mutations of F197 L and E219C | MH4 | Fed-batch | Mineral medium | 0.962 | Mora-Villalobos et al., 2018 [17] |
E. coli | Human TPH2 mutant with a deletion of first 145 N-terminal and 24 C-terminal aminoacids (TPH2, NΔ145/CΔ24) | BH4 | Fed-batch; Glycerol as carbon source | Mineral medium | 5.1 | Wang et al., 2018 [12] |
E. coli | Truncated human TPH2 (NΔ145/CΔ24) | BH4 | Shake flask; Glycerol as carbon source | Mineral medium | 1.61 | Xu et al., 2020 [43] |
E. coli | Truncated human TPH2 (NΔ145/CΔ24) with mutations of E2K, N97I and P99C | BH4 | Fed-batch; Glucose as carbon source | Mineral medium | 8.58 | This study |