Fig. 3
Whole-cell bioconversion of L-tryptophan into 5-HTP using HTP04-06 strains. a Cell growth. b 5-HTP production. c L-Trp consumption. d The ultimate conversion rate of L-Trp to 5-HTP. e Protein expression in HTP04-HTP06 strains after 12-h cultivation. M: mark; 1, 2: Soluble and inclusion expression of HTP04; 3, 4: Soluble and inclusion expression of HTP05; 5, 6: Soluble and inclusion expression of HTP06. The genotype of strains: HTP04, E. coli W3110 ∆tnaA lacI::PxylF-T7RNAP mlc::mlc* yghX::Ptrc-mtrA-PTPS-SPR yjgX::PT7-PCD-DHPR pSTV-TM; HTP05, HTP04 pSTV-TM::pSTV-TM1; HTP06, HTP04 pSTV-TM::pSTV-TM2. The data are presented as averages, and the error bars represent standard deviations (n = 3 independent experiments). ** P < 0.01