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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Strategies for efficient production of recombinant proteins in Escherichia coli: alleviating the host burden and enhancing protein activity

Fig. 2

The optimization expression strategies for decoupling the cell growth and RP production. A Manipulating the expression of RNAP subunits (β and β') or inhibiting RNAP activity by RNA polymerase inhibitor GP2 to prevent transcription of endogenous growth genes. B Inhibition of growth-related gene expression using CRISPRi. C Reducing competition for host ribosome using orthologous ribosome (O-ribosome) to specifically translate target proteins. D The uncoupling strategy allows to clearly divide an RP production process into two phases, namely the growth phase and the production phase. This allows resources to be used for RP production during fermentation

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Microbial Cell Factories

ISSN: 1475-2859

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