Table 3 Strains used in this study
Strains | Description | Source |
|---|---|---|
E. coli | ||
DH5α | For cloning and plasmid amplification purposes; wild type | Lab-owned |
Y. lipolytica | ||
Po1g ΔKU70 | MATA, xpr2-332, leu2-270, ku70-, ura3-302::URA3, Axp-2; wild type | [48] |
Po1g KC | MATA, xpr2-332, leu2-270, ku70-, ura3-302::URA3, Axp-2, codon-optimized crmas | This study |
Po1g KCE1 | MATA, xpr2-332, leu2-270, ku70-, ura3-302::URA3, Axp-2, codon-optimized crmas, erg1 | This study |
Po1g KCE9 | MATA, xpr2-332, leu2-270, ku70-, ura3-302::URA3, Axp-2, codon-optimized crmas, erg9 | This study |
Po1g KCE20 | MATA, xpr2-332, leu2-270, ku70-, ura3-302::URA3, Axp-2, codon-optimized crmas, erg20 | This study |
Po1g KCH | MATA, xpr2-332, leu2-270, ku70-, ura3-302::URA3, Axp-2, codon-optimized crmas, hmg1 | This study |
Po1g KCHE1 | MATA, xpr2-332, leu2-270, ku70-, ura3-302::URA3, Axp-2, codon-optimized crmas, HMG1, erg1 | This study |
Po1g KCHE9 | MATA, xpr2-332, leu2-270, ku70-, ura3-302::URA3, Axp-2, codon-optimized crmas, HMG1, erg9 | This study |
Po1g KCHE20 | MATA, xpr2-332, leu2-270, ku70-, ura3-302::URA3, Axp-2, codon-optimized crmas, HMG1, erg20 | This study |
Po1g KCE1E9 | MATA, xpr2-332, leu2-270, ku70-, ura3-302::URA3, Axp-2, codon-optimized crmas, erg1, erg9 | This study |
Po1g KCE1E20 | MATA, xpr2-332, leu2-270, ku70-, ura3-302::URA3, Axp-2, codon-optimized crmas, erg1, erg20 | This study |
Po1g KCE9E20 | MATA, xpr2-332, leu2-270, ku70-, ura3-302::URA3, Axp-2, codon-optimized crmas, erg9, erg20 | This study |
Po1g KCE1 | MATA, xpr2-332, leu2-270, ku70-, ura3-302::URA3, Axp-2, codon-optimized crmas, erg1 | This study |
Po1g KC-240A | MATA, xpr2-332, leu2-270, ku70-, ura3-302::URA3, Axp-2, crmasP240A | This study |
Po1g KC-243A | MATA, xpr2-332, leu2-270, ku70-, ura3-302::URA3, Axp-2, crmasF243A | This study |
Po1g KC-251A | MATA, xpr2-332, leu2-270, ku70-, ura3-302::URA3, Axp-2, crmasP251A | This study |
Po1g KC-323A | MATA, xpr2-332, leu2-270, ku70-, ura3-302::URA3, Axp-2, crmasL323A | This study |
Po1g KC-324A | MATA, xpr2-332, leu2-270, ku70-, ura3-302::URA3, Axp-2, crmasL324A | This study |
Po1g KC-323A/324A | MATA, xpr2-332, leu2-270, ku70-, ura3-302::URA3, Axp-2, crmasL323A/L324A | This study |
Po1g KC-328A | MATA, xpr2-332, leu2-270, ku70-, ura3-302::URA3, Axp-2, crmasL328A | This study |
Po1g KC-331A | MATA, xpr2-332, leu2-270, ku70-, ura3-302::URA3, Axp-2, crmasF331A | This study |
Po1g KC-341A | MATA, xpr2-332, leu2-270, ku70-, ura3-302::URA3, Axp-2, crmasC341A | This study |
Po1g KCHE9-323A | MATA, xpr2-332, leu2-270, ku70-, ura3-302::URA3, Axp-2, crmasL323A, hmg1, erg9 | This study |
Po1g KCHE20-323A | MATA, xpr2-332, leu2-270, ku70-, ura3-302::URA3, Axp-2, crmasL323A, hmg1, erg20 | This study |