Table 2 List of plasmids employed in this study

pYLEX1

Y. lipolytica integrative plasmid, pHP4D-tXPR2, leu2, Ap^r

⁴⁰

pUCCrMAS

codon-optimized (naturally occurring) crmas, Apr

Beijing Genomics Institute

pYLCrMAS

pHP4D-crmas-tXPR2, leu2, Ap^r

This study

pYLE1

pHP4D-erg1-tXPR2, leu2, Ap^r

This study

pYLE9

pHP4D-erg9-tXPR2, leu2, Ap^r

This study

pYLE20

pHP4D-erg20-tXPR2, leu2, Ap^r

This study

pYLH

pHP4D-hmg1-tXPR2, leu2, Ap^r

This study

pYLC-240A

pHP4D-crmasP240A-tXPR2, leu2, Ap^r

This study

pYLC-243A

pHP4D-crmasF243A-tXPR2, leu2, Ap^r

This study

pYLC-251A

pHP4D-crmasP251A-tXPR2, leu2, Ap^r

This study

pYLC-323A

pHP4D-crmasL323A-tXPR2, leu2, Ap^r

This study

pYLC-324A

pHP4D-crmasL324A-tXPR2, leu2, Ap^r

This study

pYLC-323A/324A

pHP4D-crmasL323A/L324A-tXPR2, leu2, Ap^r

This study

pYLC-328A

pHP4D-crmasL328A-tXPR2, leu2, Ap^r

This study

pYLC-331A

pHP4D-crmasF331A-tXPR2, leu2, Ap^r

This study

pYLC-341A

pHP4D-crmasC341A-tXPR2, leu2, Ap^r

This study

pYLCE1

pHP4D-crmas-tXPR2, pHP4D-erg1-tXPR2, leu2, Ap^r

This study

pYLCE9

pHP4D-crmas-tXPR2, pHP4D-erg9-tXPR2, leu2, Ap^r

This study

pYLCE20

pHP4D-crmas-tXPR2, pHP4D-erg20-tXPR2, leu2, Ap^r

This study

pYLCH

pHP4D-crmas-tXPR2, pHP4D-hmg1-tXPR2, leu2, Ap^r

This study

pYLCHE1

pHP4D-crmas-tXPR2, pHP4D-hmg1-tXPR2, pHP4D-erg1-tXPR2, leu2, Ap^r

This study

pYLCHE9

pHP4D-crmas-tXPR2, pHP4D-hmg1-tXPR2, pHP4D-erg9-tXPR2, leu2, Ap^r

This study

pYLCHE20

pHP4D-crmas-tXPR2, pHP4D-hmg1-tXPR2, pHP4D-erg20-tXPR2, leu2, Ap^r

This study

pYLCE1E9

pHP4D-crmas-tXPR2, pHP4D-erg1-tXPR2, pHP4D-erg9-tXPR2, leu2, Ap^r

This study

pYLCE1E20

pHP4D-crmas-tXPR2, pHP4D-erg1-tXPR2, pHP4D-erg20-tXPR2, leu2, leu2, Ap^r

This study

pYLCE9E20

pHP4D-crmas-tXPR2, pHP4D-erg9-tXPR2, pHP4D-erg20-tXPR2, leu2, leu2, Ap^r

This study

pYLCHE9-323A

pHP4D-crmasL323A-tXPR2, pHP4D-hmg1-tXPR2, pHP4D-erg9-tXPR2, leu2, Ap^r

This study

pYLCHE20-323A

pHP4D-crmasL323A-tXPR2, pHP4D-hmg1-tXPR2, pHP4D-erg20-tXPR2, leu2, Ap^r

This study