Fig. 5
Production titers of Y. lipolytica amyrin-high producers under different cultivation conditions. A Different temperatures for strains Po1g KCHE9-323A and Po1g KCHE20-323A in YPD. B Different filling volumes, FV. Strain Po1g KCHE20-323A in YPD. FV of 100 mL, 50 mL and 25 mL correspond to kLa of 16 h−1, 39 h−1 and 84 h−1, respectively. C Different media formulation. YPD: yeast-peptone-glucose; YPD(Mg): YPD supplemented with magnesium sulphate; YPO: yeast-peptone-waste cooking oil. Amyrins were quantified after 5 days of cultivation in shake flasks. All values presented are the mean of three biological replicates ± standard deviation