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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Enhanced production of amyrin in Yarrowia lipolytica using a combinatorial protein and metabolic engineering approach

Fig. 3

Close-up perspective of the predicted CrMAS substrate binding cluster, Site 1, visualized using PyMOL. A 2-D representation of the substrate access tunnel, of wild-type CrMAS. Hydrophobic amino acids in green, polar amino acids in blue, positively charged amino acids in purple, negatively charged amino acid residues in red. 3-D models of the substrate access tunnel of B wild-type CrMAS, C mutated CrMAS, and D superimposed of both. Site-directed mutagenesis were performed to change the eight target amino acids to alanine, as indicated in pink. Molecular structure of 2,3-oxidosqualene is displayed in cyan

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Microbial Cell Factories

ISSN: 1475-2859

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