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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Enhanced production of amyrin in Yarrowia lipolytica using a combinatorial protein and metabolic engineering approach

Fig. 2

Triterpenoid titers in the engineered Y. lipolytica in YPD. Prevailing genes in the MVA pathway, namely HMG1, ERG20, ERG9 and ERG1, were overexpressed singly and in combination. A Amyrin titers with single gene overexpression. B Squalene titers with single gene overexpression. C Amyrin titers with multiple gene overexpression. D Squalene titers with multiple gene overexpression. Control strain: Po1g ΔKU70. The codon-optimized CrMAS gene was used in all the engineered strains. Amyrins were quantified after 5 days of cultivation in shake flasks. Each engineered strain was named based on the genes that were overexpressed. The codon-optimized CrMAS gene: C; HMG1 gene: H; ERG1 gene: E1; ERG9 gene: E9; ERG20 gene: E20. For example, the Po1g KCHE20 strain refers to Y. lipolytica Po1g ΔKU70 overexpressing CrMAS, HMG1 and ERG20. All values presented are the mean of three biological replicates ± standard deviation

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Microbial Cell Factories

ISSN: 1475-2859

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