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Table 5 Primers used for PCR amplification of DNA encoding fragments of human CB1 receptor cytosolic tail

From: Design and validation of recombinant protein standards for quantitative Western blot analysis of cannabinoid CB1 receptor density in cell membranes: an alternative to radioligand binding methods

Encoded peptide

Forward primers (5′- > 3′)

Reverse primers (5′- > 3′)

CB1414–472

G^GATCCTGTGAAGGCACTGCGCAGC

GC^GGCCGCTCACAGAGCCTCGGCAGACGTGTC

CB1414–442

G^GATCCTGTGAAGGCACTGCGCAGC

GC^GGCCGCTCAAACACTGGCTGCATTGTTTGC

  1. BamHI and NotI restriction sites are highlighted in bold. The ^ symbols indicate the cleavage sites for restriction enzymes. Underlined TCA sequences represent translation stop sites
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Microbial Cell Factories

ISSN: 1475-2859

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