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Table 4 Results of radioligand saturation binding assays performed on P2 membranes of rat brain cortex

From: Design and validation of recombinant protein standards for quantitative Western blot analysis of cannabinoid CB1 receptor density in cell membranes: an alternative to radioligand binding methods

  1. [3H]-SR141716A and [3H]-CP55,940 are inverse agonist and full agonist for the CB1 receptor, respectively. Bmax, maximum number of binding sites. KD, affinity. ∆Bmax, percent increase in Bmax for radioligand [3H]-SR141716A after addition of 100 µM GTPγS relative to control (no addition) condition
  2. *p < 0.05, *** p < 0.001, one-way ANOVA with Bonferroni post hoc test. Data are mean ± SEM of three independent experiments (n = 3)
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Microbial Cell Factories

ISSN: 1475-2859

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